HEALING OF BROKEN LINEAR DICENTRIC CHROMOSOMES IN YEAST
نویسندگان
چکیده
منابع مشابه
Resolution of dicentric chromosomes by Ty-mediated recombination in yeast.
We have integrated a plasmid containing a yeast centromere, CEN5, into the HIS4 region of chromosome III by transformation. Of the three transformant colonies examined, none contained a dicentric chromosome, but all contained a rearranged chromosome III. In one transformant, rearrangement occurred by homologous recombination between two Ty elements; one on the left arm and the other on the righ...
متن کاملPreferential Breakpoints in the Recovery of Broken Dicentric Chromosomes in Drosophila melanogaster.
We designed a system to determine whether dicentric chromosomes in Drosophila melanogaster break at random or at preferred sites. Sister chromatid exchange in a Ring-X chromosome produced dicentric chromosomes with two bridging arms connecting segregating centromeres as cells divide. This double bridge can break in mitosis. A genetic screen recovered chromosomes that were linearized by breakage...
متن کاملInstability of dicentric plasmids in yeast.
Dicentric plasmids containing either two copies of centromere 4 or one copy of centromere 4 and one copy of centromere 3 in the yeast plasmid vector YRp17 were constructed in vitro and introduced into yeast cells by DNA transformation. The resulting colonies were heterogeneous for a mixed population of rearranged plasmids. The rearrangements always involved deletion of one or both centromere se...
متن کاملMeiotic and mitotic behavior of dicentric chromosomes in Saccharomyces cerevisiae.
Meiotic recombination between a circular and a linear chromosome in Saccharomyces cerevisiae has been investigated. The circle was a haploid-viable derivative of chromosome III constructed by joining regions near the two chromosome ends via a recombinant DNA construction: (HMR/MAT-URA3-pBR322-MAT/HML) and was also deleted for MAL2 (which therefore uniquely marks a linear chromosome III). Recomb...
متن کاملSir protein–independent repair of dicentric chromosomes in Saccharomyces cerevisiae
Sir2 protein has been reported to be recruited to dicentric chromosomes under tension, and such chromosomes are reported to be especially vulnerable to breakage in sir2Δ mutants. We found that the loss of viability in such mutants was an indirect effect of the repression of nonhomologous end joining in Sir(-) mutants and that the apparent recruitment of Sir2 protein to chromosomes under tension...
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ژورنال
عنوان ژورنال: Genetics
سال: 1984
ISSN: 1943-2631
DOI: 10.1093/genetics/106.2.207